RA I plus; Troubleshoots procedures if experimental results are unexpected or quality of specimens is poor. May suggest alternative routine procedures to crosscheck own findings. Performs techniques typically required for the laboratory/study. RA I plus. Assists with planning specific research procedures, coordinating schedules of experiments and procedures. Develops and troubleshoots new techniques specific to the lab. RA I plus. Maintains accurate records of results and methodologies into a log of work performed. Compiles and helps analyze data, preparing charts, graphs, and results of studies. RA I plus. Demonstrates basic laboratory techniques to laboratory assistants and technicians, research fellows, residents and students. Contributes to the team within and between departments and organizations.

Principal Summary
At Boston Children’s Hospital, we strive to develop new treatments for pediatric disease. We are seeking a highly motivated and independent individual as a research assistant to join our basic and translational research group in the Department of Cardiology at Boston Children’s Hospital. The research assistant will be a member of a team that is developing novel therapies for inherited heart disease.

The Pu lab is a multi-disciplinary lab that studies heart development and disease using advanced molecular and cellular approaches in animal models and human stem cells. Lab members are exposed to a diverse array of techniques and research ranging from fundamental, mechanistic studies of gene regulation to translational development of novel therapies.

Key Responsibilities

• Culturing stem cells and differentiating them into cardiomyocytes and other cell types.
• Reprogramming cells to pluripotency.
• Genome editing of stem cells.
• Studies using stem cells to model heart disease.
• Maintaining excellent records of results and methodologies as well assisting in compiling and analyzing data.

Minimum Qualifications
Education:

• The knowledge of theories, principles, and concepts acquired through completion of a bachelor’s degree in biology, animal science, toxicology, pharmacology or a related field. A Master’s degree is desired but not required.

Experience:

• At least 12 months of hands-on laboratory experience.
• Experience with mammalian cell culture is required.
• Intermediate laboratory skills including cell culture, molecular biology, microscopy, and use of basic analytical equipment are required.
• Excellent inter-personal skills and the desire to learn new skills are a must.
• Excellent record-keeping is a must.
• Opportunities for advancement and clinical observation will be available.